Anti-inflammatory and antioxidant effects of using alpha-tocopherol in cell culture of the parotid gland under conditions similar to diabetes mellitus
Background and aims: Hyperglycemia is one of the most common causes that leads to oxidative damage, is frequently observed in association with inflammatory infiltration mediated by mononuclear phagocytes and epithelial cells present in glandular tissues of diabetic patients. Actions that make it possible to reduce tissue damage caused by the oxidative process inherent in this metabolic disease can become an efficient alternative in helping to maintain the quality of life of millions of people. The present study aimed to analyze the effects of the use of alpha-tocopherol in cultures of primary cells of the parotid gland submitted to conditions similar to those that occur in diabetes mellitus. Methods: For this, cells from the parotid glands of Balb / C / Unib mice were extracted using the Percoll® protocol. The cells were organized into five groups with different amounts of glucose diluted in the cell culture medium and with the presence or absence of lipopolysaccharide to induce the inflammatory process, as well as the presence or absence of alpha-tocopherol. Results: The observed results indicated an increase in cell viability with the use of alpha-tocopherol and reductions of this same variable when in the presence of oxidizing agents. The proteins involved with the mediation of antioxidant protection and inflammation, NRF2, nuclear factor kappa B, and glutathione peroxidase were increased in the groups submitted to a hyperglycemic and inflammatory condition, but the same behavior was not observed in the other groups of the experiment. Conclusion: It is concluded, therefore, that alpha-tocopherol showed anti-inflammatory and antioxidant effects in this type of cell culture, attenuating the harmful effects of the conditions in which the cells were subjected.