EFFECT OF BREWED ROBUSTA COFFEE LEAVES ON INSULIN LEVELS AND HOMA-IR INDEX IN METABOLIC SYNDROME RATS

Nurmasari Widyastuti; Gemala Anjani; Vita Gustin Almira; Suci Eka Putri; Amali Rica Pratiwi; M Iqbal Prawira-Atmaja

Nurmasari Widyastuti Universitas Diponegoro
Gemala Anjani
Vita Gustin Almira
Suci Eka Putri
Amali Rica Pratiwi
M Iqbal Prawira-Atmaja

Keywords: metabolic syndrome, Robusta coffee leaves, Insulin Levels, HOMA-IR Index

Abstract

Introduction: Central obesity and insulin resistance have been known as the main risk factors for metabolic syndrome. Robusta coffee leaves could be a functional food to prevent metabolic syndrome with phytochemical compounds. This study aimed to prove the effectiveness of the administration of brewed Robusta coffee leaves on insulin levels and HOMA-IR index in metabolic syndrome rats. Material and Methods: Male Wistar rats (6 weeks old, 150-200 g, n = 36) were divided into six groups (n = 6 rats/group): normal control group (K1), metabolic syndrome control group without treatment (K2), mangiferin treatment group (X1), brewed Robusta coffee leaves 0.09 g/200BW group (X2), brewed Robusta coffee leaves 0.18 g/200BW group (X3), brewed Robusta coffee leaves 0.36 g/200BW group (X4). Besides the K1 group, the other groups were given a high-fat-fructose diet. Each dose of coffee leaves was brewed with 3.6 mL of water at 70°C for 10 minutes. The intervention was given for 28 days. Results: There was a significant increase in insulin levels (p<0.000) in all groups and a significant decrease in the HOMA-IR index in treatment groups (p<0.000). Conclusions: The administration of brewed Robusta coffee leaves increased insulin levels, and decreased the HOMA-IR index in metabolic syndrome rats with the most effective dose being 0.36 g/200gBW.