EVALUATION OF PANCREATIC AND EXTRA PANCREATIC EFFECTS OF BRANCHED AMINO ACIDS

  • Moath Alqaraleh (assistant professor of Molecular biology and biochemistry) Department of Biology Sciences, faculty of Science. The University of Jordan, Amman, Jordan.
  • Violet Kasabri (professor of clinical biochemistry). Department of Pharmacy, Faculty of Pharmacy. The University of Jordan, Amman, Jordan
  • Sundus Al alaw (master in biology ) . Department of Biology Sciences, faculty of Science. The University of Jordan, Amman, Jordan
  • Nihad Al-Othman (assistant professor of Molecular biology) An-Najah National University, Nablus, Palestine
Keywords: BCAAs, insulin secretion, beta cells proliferation, amylase, glucosidase and lipase

Abstract

Background and aims: Leucine, Isoleucine, and Valine collectively known as Branched-chain amino acids (BCAAs), can be closely associated with metabolic dysregularities and with insulin resistance. We aimed to explore the role of BCAAs as potential treatment option for diabetes. Material and method: Bioassay the effect of BCAAs on MIN6 cell line on insulin secretion and pancreatic beta cells expansion, then were checked for inhibitory potential of pancreatic amylase, glucosidase and lipase as alternative approach for diabetes treatment. Results: BCAAs significantly enhance insulin secretion parallel to L-alanine efficacy. Furthermore, BCAAs obtain a dose dependent β-cell proliferation similar to glucagon-like peptide-1. Moreover, these acids could restore the secretory function of MIN6 β-cell despite stressful gluco-lipo-toxicity; separately or combined. Moreover, BCAAs exerted a dose dependent dual inhibition of amylase, glucosidase and lipase. Conclusions: Our current findings suggest that BCAAs supplementation may have a potential therapeutic effect against diabetes as insulin releasing agent and as specific inhibitors for both -amylase/α-amyloglucoside and lipase

Published
2019-06-30
How to Cite
Alqaraleh, Moath, Violet Kasabri, Sundus Al alaw, and Nihad Al-Othman. 2019. “EVALUATION OF PANCREATIC AND EXTRA PANCREATIC EFFECTS OF BRANCHED AMINO ACIDS”. Romanian Journal of Diabetes Nutrition and Metabolic Diseases 26 (2), 199-209. http://www.rjdnmd.org/index.php/RJDNMD/article/view/618.
Section
Original Research Articles