GENDER-SPECIFIC DIFFERENCES OF OXIDATIVE PROCESSES IN THE POPULATION OF CIRCULATING NEUTROPHILS OF RATS IN A SETTING OF PROLONGED ADMINISTRATION OF MONOSODIUM GLUTAMATE
Abstract
Background and aims: Monosodium salt of glutamic acid (MSG) is one of the most common food additives in Ukraine and in Europe with global production over 200 thousand tons annually. The aim of our study was to assess, in gender-specific terms, how prolonged administration of monosodium glutamate effects on reactive oxygen and nitrogen species formation and the apoptotic/necrotic processes in the population of circulating neutrophils of rats. Materials and methods: Experimental tests were conducted in 32 nonlinear, mature white rats. MSG was administered intragastrically at a dose of 30 mg/kg body weight for 30 days. The analysis of cell samples to determine neutrophils with overproduction of ROS and signs of apoptosis\necrosis was evaluated with flow laser cytometry method. The total NOS activity was determined by monitoring the rate of conversion of L-arginine into citrulline. The total quantity of NO metabolites was assessed by evaluating their amount, which included nitrite ions that were initially present in the sample (NO2–) and nitrate ions reducted to nitrites (NO3–). Results: We found a significant increase in generation of reactive oxygen species by circulating neutrophils, intensification of nitroxydergic processes both in serum and in lung homogenate, an increase in the percentage of circulating neutrophils with signs of apoptosis and no changes in the percentage of circulating neutrophils with signs of necrosis. Conclusions: We observed activation of oxidative and nitroxydergic processes in rats with prolonged administration of monosodium glutamate, which initiate apoptosis. In gender-specific terms, a more pronounced changes were seen in male rats.
