Some vital enzymes in Dupuytren’s disease and diabetes mellitus as a risk factor

Gayane Hekimyan; Sona Mardanyan; Artavazd Sahakyan; Luiza Karapetyan; Alvard Antonyan

Gayane Hekimyan Department of Metabolism of Adenylic Compounds, H. Buniatian Institute of Biochemistry of Armenian NAS, Yerevan, Armenia
Sona Mardanyan Department of Metabolism of Adenylic Compounds, H. Buniatian Institute of Biochemistry of Armenian NAS, Yerevan, Armenia
Artavazd Sahakyan Department of Metabolism of Adenylic Compounds, Yerevan State Medical University, Yerevan, Armenia
Luiza Karapetyan Department of Metabolism of Adenylic Compounds, H. Buniatian Institute of Biochemistry of Armenian NAS, Yerevan, Armenia
Alvard Antonyan Department of Metabolism of Adenylic Compounds, H. Buniatian Institute of Biochemistry of Armenian NAS, Yerevan, Armenia

Keywords: adenosine deaminase 1, adenosine deaminase 2, aponeurosis, dipeptidyl peptidase 4, dipeptidyl peptidase 2

Abstract

Dupuytren’s disease (DD) is a disease of unknown etiology, when the finger remains in a flexed position due to thickening of the aponeurosis. Our study aimed to investigate a possible relationship between adenosine deiminases 1 and 2 (ADA1, ADA2) and dipeptidyl peptidases 4 and 2 (DPP4, DPP2) with DD. The activity of named enzymes in aponeurosis and blood plasma from patients with DD and with various hand injuries (healthy individuals, HI) were compared. In aponeurosis from DD patients, ADA1 and ADA2 activities were higher by 5.6 and 7.8 times, respectively, compared to aponeurosis from HI. High levels of DPP4 and DPP2 activity were recorded in the DD aponeurosis, whereas they were untestable in the HI aponeurosis. Their activity in blood plasma was ~1.5 times higher in DD than HI subjects. In the aponeurosis, the highest activity of ADA1 was in sever level of DD, while of DPP4 and DPP2 in moderate level. ADA1 and DPP4 activity were significantly higher in DD with diabetes than without it. ADA and DPP can be recommended as targets in the fight against DD using their inhibitors assumed in medicine, which can help to avoid surgical intervention.