Genetic and nutritional interaction: impact of isomaltooligosaccharide and PRKAA2 & ABCA1 polymorphism on Castelli’s risk index

Gharini Sumbaga Narhadina; Sunarti Sunarti; Arta Farmawati

Gharini Sumbaga Narhadina Department of Biochemistry, Master of Biomedical Science, Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University, Yogyakarta, Indonesia
Sunarti Sunarti Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Yogyakarta, Indonesia
Arta Farmawati Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University, Yogyakarta, Indonesia

Keywords: cardiac index, oligosaccharide, ABCA, PRKAA2, hyperlipidemias

Abstract

Castelli’s Risk Index 1 (CRI1) can be used to estimate the risk of developing ischemic heart disease, which can be influenced by hyperlipidemia. Both genetic variants, including ABCA1 rs2066714 and PRKAA2 rs1124900, and also consuming isomaltooligosaccharide (IMO) can affect hyperlipidemia. The study’s objectives are to examine the alterations in CRI1 in hyperlipidemia subject who eat IMO cookies and have genetic differences. 30 DNA samples were taken from hyperlipidemic participants who received a 4-week treatment of IMO cookies and a control. Genetic variation analysis using PCR-RFLP and restriction enzymes digestion (AfiIII for PRKAA2 rs1124900 and EcoRV for ABCA1 rs2066714). Consumption of IMO significantly reduced CRI1 (8.06%) in patients with hyperlipidemia (p<0.05). Interaction genotypes TG and GG of PRKAA2 rs1124900 with IMO consumption lowering CRI1 (p<0.05). In conclusion, consuming IMO cookies can lower the risk of CRI1 in those with TG and GG genotypes of PRKAA2 rs1124900.