Prognostic value of the level of expression of cell wall proteins and cytokines during inflammation in tumor tissue culture in patients with type 2 diabetes mellitus

Oleksii Volodimirovich Movchan; Irina Yuriivna Bagmut; Michael Ivanovich Sheremet; Lidia Anatolyivna Sukhanova; Igor Leonidovich Kolisnyk; Oleksandr Vasyliovych Bagmut; Olexksii Oleksandrovich Halmiz; Olena Valeriyivna Havrish; Andriy Oleksandrovich Lyashenko; Irina Viktorivna Dosenko; Oksana Mykolaivna Ivankova; Anton Dmitrovich Loboda; Oleksandr Volodimirovich Lazaruk

Oleksii Volodimirovich Movchan National Cancer Institute of the Ministry of Health of Ukraine, Kiyv, Ukraine
Irina Yuriivna Bagmut Department of Infection Control, Kharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, Ukraine
Michael Ivanovich Sheremet Department of Surgery No.1, Bukovinian State Medical University, Chernivtsi, Ukraine
Lidia Anatolyivna Sukhanova Department of Infection Control, Kharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, Ukraine
Igor Leonidovich Kolisnyk Department of Infection Control, Kharkiv National Medical University of the Ministry of Health of Ukraine, Kharkiv, Ukraine
Oleksandr Vasyliovych Bagmut Faculty of Computer Sciences, Karazin Kharkiv National University, Kharkiv, Ukraine
Olexksii Oleksandrovich Halmiz Department of Neurology, Narcology, Psychiatry, Ophthalmology, Otolaryngology and Oncology of Luhansk State Medical University, Rivne, Ukraine
Olena Valeriyivna Havrish Department of Neurology, Narcology, Psychiatry, Ophthalmology, Otolaryngology and Oncology of Luhansk State Medical University, Rivne, Ukraine
Andriy Oleksandrovich Lyashenko National Cancer Institute of the Ministry of Health of Ukraine, Kiyv, Ukraine
Irina Viktorivna Dosenko National Cancer Institute of the Ministry of Health of Ukraine, Kiyv, Ukraine
Oksana Mykolaivna Ivankova National Cancer Institute of the Ministry of Health of Ukraine, Kiyv, Ukraine
Anton Dmitrovich Loboda National Cancer Institute of the Ministry of Health of Ukraine, Kiyv, Ukraine
Oleksandr Volodimirovich Lazaruk Department of Pathology, Bukovinian State Medical University, Chernivtsi, Ukraine

Keywords: chitinase-like proteins, interleukins, tumor tissue culture, inflammatory breast cancer, diffuse stomach and diffuse esophagus cancer, type 2 diabetes mellitus

Abstract

The levels of chitinase-like proteins in circulating blood increase with inflammatory types of tumors.The aim of the study aimed to prognostic value of expression of chitinase-like proteins and interleukins levels in tumor tissue culture in patients with inflammatory breast cancer, diffuse stomach and diffuse esophagus cancer with amplification erBb2 (3+) with type 2 diabetes mellitus. We determined the expression of CD68, a marker of M2 subpopulation RS1 (stabilin-1), chitinase-like proteins YKL-39 i SI-CLP and interleukins levels on comparison of amplification erBb2 in patients with Inflammatory Breast Cancer (IBC), Diffuse Stomach (DSC) and Diffuse Esophagus Cancer (DEC). In all patients were performed conditions of plasma glucose levels after operation. Ist block: 21 patients with a confirmed mutation of the Her-2/neu gene and amplification erBb2 (3+) with type 2 diabetes mellitus: IBC 7, DSC 7 and DEC 7. IInd (block): 21 patients IBC 7, DSC 7 and DEC 7, without amplification erBb2 and normal range of plasma glucose level. As a model for tumor-associated macrophages: stimulation of human primary macrophages by cytokine M2-polarization of IL-4 and tumor cell supernatants (CSF2RA for breast cancer, and Colo206-M for stomach and esophagus cancer). With overexpression, erBb2 and serum markers increase for DSC and DEC by 36% (P<0.005). The immune microenvironment in the tissue of the tumors is manifested by higher levels of IL-1β and IL-8 (3 times), lower levels of IL-18 (2 times) and higher density of CD3 + (10 times). Thus, studies have shown that patients with type 2 diabetes mellitus operated on for inflammatory cancers are formed with deeper immune deficiency.